WEB Figure 1.







Web Fig1. Confirmation of Blimp-1 Target Genes in Human Cell Lines. (A) RT-PCR analysis of Blimp-1 Targets. BJAB cell were acutely infected with control (puro) or Blimp-1-expressing (blimp-1) virus, selected with puromycin, and RNA was harvested for conversion to cDNA and RT-PCR analysis. GAPDH amplification was used as a control. (B) Western blotting of potential Blimp-1 targets in control (puro) and Blimp-1 expressing (blimp-1) BJAB cells. SP1 expression is used as the control. (C) Flow cytometric analysis of Blimp-1 target genes that are cell surface markers in BJAB. BJAB cells were infected in parallel with control (puro) of Blimp-1 (Blimp-1/puro) viruses. After 24hrs, puromycin was added, and after an additional 48hrs selected cells were harvested for flow cytometry and stained as described below. (D) Flow cytometric analysis of Blimp-1 target genes that are cell surface markers in RAJI, and WI-L2 cells were infected with viruses carrying control (gfp only, green trace) or Blimp-1 plus gfp (red trace). After 24 hrs. cells were gated for gfp expression and counterstained with the appropriate PE-conjugated antibody against a cell surface protein. Background staining with a control antibody is shown in black.